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EVI2B Polyclonal antibody

EVI2B Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24891-1-AP

Synonyms

CD361, D17S376, EVDB, EVI 2B, EVI2B, Protein EVI2B

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EVI2B Polyclonal antibody (24891-1-AP)

    WB analysis of HepG2 using 24891-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24891-1-AP (EVI2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using EVI2B Polyclonal antibody (24891-1-AP)

    WB analysis of A549 using 24891-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24891-1-AP (EVI2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EVI2B Polyclonal antibody (24891-1-AP)

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EVI2B Polyclonal antibody (24891-1-AP)

    IHC staining of human tonsillitis using 24891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24891-1-AP (EVI2B Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of Jurkat cells using EVI2B Polyclonal antibody (24891-1-AP)

    FC experiment of Jurkat using 24891-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug EVI2B antibody (24891-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHepG2 cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

The immunogen of 24891-1-AP is EVI2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EVI2B fusion protein Ag19511
Full Name ecotropic viral integration site 2B
Calculated Molecular Weight 448 aa, 49 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC005926
Gene Symbol EVI2B
Gene ID (NCBI) 2124
RRIDAB_2879781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EVI2B antibody 24891-1-APDownload protocol
IHC protocol for EVI2B antibody 24891-1-APDownload protocol
FC protocol for EVI2B antibody 24891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma.

Authors - Bingsheng Yang
View Article
humanIHC

Exp Biol Med (Maywood)

Analysis of scRNA-seq and bulk RNA-seq demonstrates the effects of EVI2B or CD361 on CD8+ T cells in osteosarcoma

Authors - Tianyu Xie
View Article