ADAP2 Polyclonal antibody

ADAP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13706-1-AP

Synonyms

ADAP2, ArfGAP with dual PH domains 2, cent b, CENTA2, Centaurin alpha 2, Cnt a2, HSA272195

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Validation Data Gallery

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  WB analysis of human placenta using 13706-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13706-1-AP (ADAP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13706-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13706-1-AP (ADAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 13706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13706-1-AP (ADAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 13706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13706-1-AP (ADAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse liver tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13706-1-AP targets ADAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADAP2 fusion protein Ag4641
• Full Name: ArfGAP with dual PH domains 2
• Calculated Molecular Weight: 380 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC033758
• Gene Symbol: ADAP2
• Gene ID (NCBI): 55803
• RRID: AB_10644119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.