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JAB1 Polyclonal antibody

JAB1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27511-1-AP

Synonyms

COPS5, CSN5, JAB1, MOV 34, SGN5, Signalosome subunit 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAB1 Polyclonal antibody (27511-1-AP)

    WB analysis using 27511-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27511-1-AP (JAB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using JAB1 Polyclonal antibody (27511-1-AP)

    IHC staining of mouse liver using 27511-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27511-1-AP (JAB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using JAB1 Polyclonal antibody (27511-1-AP)

    IHC staining of rat liver using 27511-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27511-1-AP (JAB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JAB1 Polyclonal antibody (27511-1-AP)

    IF Staining of HeLa using 27511-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JAB1 antibody (27511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using JAB1 Polyclonal antibody (27511-1-AP)

    IF Staining of HeLa using 27511-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using JAB1 antibody (27511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using JAB1 Polyclonal antibody (27511-1-AP)

    FC experiment of HEK-293 using 27511-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human JAB1 (27511-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inmouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27511-1-AP targets JAB1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAB1 fusion protein Ag25950
Full Name COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC001187
Gene Symbol COPS5
Gene ID (NCBI) 10987
RRIDAB_2880894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for JAB1 antibody 27511-1-APDownload protocol
IHC protocol for JAB1 antibody 27511-1-APDownload protocol
IF protocol for JAB1 antibody 27511-1-APDownload protocol
FC protocol for JAB1 antibody 27511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Oncol

Identification of Key Transcription Factors and Immune Infiltration Patterns Associated With Breast Cancer Prognosis Using WGCNA and Cox Regression Analysis.

Authors - Xin Yin
View Article
humanIF

Mol Carcinog

PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma

Authors - Qiang Li
View Article
humanWB

Cancer Cell Int

The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.

Authors - Huilin Zhang
View Article
mouseWB

Neuropsychiatr Dis Treat

COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.

Authors - En Liang
View Article
humanWB,IHC,IF

Cell Commun Signal

Alpha5 nicotinic acetylcholine receptor mediated immune escape of lung adenocarcinoma via STAT3/Jab1-PD-L1 signalling

Authors - Ping Zhu
View Article