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LSAMP Polyclonal antibody

LSAMP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13600-1-AP

Synonyms

IgLON family member 3, IGLON3, LAMP, LSAMP

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using LSAMP Polyclonal antibody (13600-1-AP)

    WB analysis of human brain using 13600-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13600-1-AP (LSAMP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LSAMP Polyclonal antibody (13600-1-AP)

    WB analysis of mouse brain using 13600-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13600-1-AP (LSAMP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13600-1-AP targets LSAMP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSAMP fusion protein Ag4542
Full Name limbic system-associated membrane protein
Calculated Molecular Weight 338 aa, 37 kDa
Observed Molecular Weight 64-68 kDa
GenBank Accession NumberBC033803
Gene Symbol LSAMP
Gene ID (NCBI) 4045
RRIDAB_10597712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSAMP, also known as LAMP, is a 64-68 kDa heavily glycosylated neural cell adhesion molecule expressed on the neuronal dendrites and somata (PMID: 1688937; 8666243). LSAMP is a member of the IgLON family. The amino acid sequence of LSAMP is highly conserved among species. Human LSAMP shares 99% amino acid sequence identity with rodent LSAMP. The impact of LSAMP protein on neurite outgrowth and neuronal connectivity has been established in a wide spectrum of psychiatric disorders in humans (PMID: 26136648). In mice, lack of LSAMP protein seemingly leads to a deterioration in the ability to adapt to novel stressful environments and stimuli (PMID: 22155487).

Protocols

Product Specific Protocols
WB protocol for LSAMP antibody 13600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols