MAG Polyclonal antibody

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  WB analysis using 14386-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spinal cord using 14386-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 14386-1-AP (MAG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14386-1-AP (MAG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14386-1-AP (MAG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 14386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using MAG antibody (14386-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Histone H3 antibody (68345-1-Ig, Clone: 1A2A3, blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAG Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 14386-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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Tested Applications

• Positive WB detected in: mouse spinal cord tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

14386-1-AP targets MAG in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5845

  Product name: Recombinant human MAG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 199-516 aa of BC053347

  Sequence: VSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP

• Full Name: myelin associated glycoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC053347
• Gene Symbol: MAG
• Gene ID (NCBI): 4099
• RRID: AB_2878051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20916
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Brain; A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination.; Authors - Tuancheng Feng
mouse	WB	Acta Neuropathol Commun; TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.; Authors - Tuancheng Feng
mouse	WB,IF	J Cereb Blood Flow Metab; Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.; Authors - Weiwei Yu
mouse	WB	CNS Neurosci Ther; The Neuroprotective Mechanisms of PPAR-γ: Inhibition of Microglia-Mediated Neuroinflammation and Oxidative Stress in a Neonatal Mouse Model of Hypoxic-Ischemic White Matter Injury; Authors - Mingchu Fang
	WB	Mol Neurobiol; FGF21 Alleviates Hypoxic-Ischemic White Matter Injury in Neonatal Mice by Mediating Inflammation and Oxidative Stress Through PPAR-γ Signaling Pathway; Authors - Mingchu Fang
mouse	WB	Mol Neurobiol; Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.; Authors - Zihu Tan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (02-22-2022)

Works well on FFPE prostate tissue by IF. (Tris-EDTA antigen retrieval).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate