Tested Applications
| Positive WB detected in | mouse spinal cord tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14386-1-AP targets MAG in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5845 Product name: Recombinant human MAG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 199-516 aa of BC053347 Sequence: VSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP Predict reactive species |
| Full Name | myelin associated glycoprotein |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC053347 |
| Gene Symbol | MAG |
| Gene ID (NCBI) | 4099 |
| RRID | AB_2878051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAG antibody 14386-1-AP | Download protocol |
| IHC protocol for MAG antibody 14386-1-AP | Download protocol |
| WB protocol for MAG antibody 14386-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAG (myelin associated glycoprotein) polyclonal antibody (Cat# 14386-1-AP) has 34 citations spanning journals including Nature Communications, Science Advances, Brain, Cell Reports, J Neuroinflammation, Aging Cell, CNS Neuroscience & Therapeutics, Experimental Neurology, and Molecular Neurobiology. Research fields cover myelination/demyelination, white matter injury, neuroinflammation, spinal cord injury, ischemic stroke, oligodendrocyte development, neurodegeneration, and ferroptosis, with applications primarily in WB, IF, and IHC across human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Nat Commun Huntington disease oligodendrocyte maturation deficits revealed by single-nucleus RNAseq are rescued by thiamine-biotin supplementation | ||
Adv Sci (Weinh) The E3 Ubiquitin Ligase RLIM Safeguards Oligodendrocyte Development and Myelination by Targeting SLC7A11 for Polyubiquitination to Regulate Ferroptotic Resistance | ||
Sci Adv RNF220-mediated K63-linked polyubiquitination stabilizes Olig proteins during oligodendroglial development and myelination | ||
Brain A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination. | ||
J Neuroinflammation SIRT6 modulates lesion microenvironment in LPC induced demyelination by targeting astrocytic CHI3L1 | ||
Aging Cell Intermittent Fasting Enhances Motor Coordination Through Myelin Preservation in Aged Mice |
Reviews
What customers say
The single review reports successful use of this antibody for immunofluorescence on FFPE prostate tissue using Tris-EDTA antigen retrieval at a 1:50 dilution, with a 5-star rating. Overall, customers find it performs well for IF applications on formalin-fixed paraffin-embedded tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emma (Verified Customer) (02-22-2022) | Works well on FFPE prostate tissue by IF. (Tris-EDTA antigen retrieval).
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