AF-6 Polyclonal antibody

AF-6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55102-1-AP

Synonyms

AF 6, AF6, AFADIN, Afadin variant, MLLT4



Tested Applications

Positive WB detected inmouse kidney tissue, A431 cells, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

55102-1-AP targets AF-6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4
Calculated Molecular Weight 207 kDa
Observed Molecular Weight 182-210 kDa
GenBank Accession NumberNM_005936
Gene Symbol Afadin/AF6
Gene ID (NCBI) 4301
RRIDAB_10792809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLLT4, also named as AF6 and Afadin, belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It is a nNectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. The antibody is specific to MLLT4.

Protocols

Product Specific Protocols
WB protocol for AF-6 antibody 55102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice

Authors - Deling Wu
ratWB

Res Sq

Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance

Authors - Joel James
mouseWB

Aging (Albany NY)

The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level

Authors - Wei Wang