PPIG Polyclonal antibody

PPIG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12985-1-AP

Synonyms

Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin



Tested Applications

Positive WB detected inhuman liver tissue, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

12985-1-AP targets PPIG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIG fusion protein Ag3622
Full Name peptidylprolyl isomerase G (cyclophilin G)
Calculated Molecular Weight 739 aa, 87 kDa
Observed Molecular Weight 89 kDa
GenBank Accession NumberBC001555
Gene Symbol PPIG
Gene ID (NCBI) 9360
RRIDAB_2169281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPIG antibody 12985-1-APDownload protocol
IHC protocol for PPIG antibody 12985-1-APDownload protocol
IF protocol for PPIG antibody 12985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

High-throughput tagging of endogenous loci for rapid characterization of protein function

Authors - Joonwon Kim