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OAT3/SLC22A8 Polyclonal antibody

OAT3/SLC22A8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16844-1-AP

Synonyms

hOAT3, OAT3, Organic anion transporter 3, SLC22A8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OAT3/SLC22A8 Polyclonal antibody (16844-1-AP)

    WB analysis using 16844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16844-1-AP (SLC22A8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16844-1-AP targets OAT3/SLC22A8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OAT3/SLC22A8 fusion protein Ag9795
Full Name solute carrier family 22 (organic anion transporter), member 8
Calculated Molecular Weight 542 aa, 60 kDa
Observed Molecular Weight70 kDa
GenBank Accession Numberbc022387
Gene Symbol SLC22A8
Gene ID (NCBI) 9376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OAT3 (encoded by SLC22A8) is widely distributed in the kidney, liver, choroid plexus, olfactory mucosa, brain, retina, and placenta, but it is pre-dominantly expressed on the basolateral membrane of renal tubular cells. OAT3 plays a crucial role in the uptake, distribution, and excretion of various endogenous/exogenous substances. 16844-1-AP detected ~70 kDa is greater than predicted for Oat3 protein containing 542 amino acid (calculated Mr 59 kDa) and probably represents the glycosylated form (PMID:23389457).

Protocols

Product Specific Protocols
WB protocol for OAT3/SLC22A8 antibody 16844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols