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VTA1 Polyclonal antibody

VTA1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15786-1-AP

Synonyms

HSPC228, DRG-1, DRG1, DRG 1, Dopamine-responsive gene 1 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VTA1 Polyclonal antibody (15786-1-AP)

    WB analysis using 15786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VTA1 Polyclonal antibody (15786-1-AP)

    WB analysis using 15786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using VTA1 Polyclonal antibody (15786-1-AP)

    WB analysis of HepG2 using 15786-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using VTA1 Polyclonal antibody (15786-1-AP)

    WB analysis of K-562 using 15786-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human urine sample using VTA1 Polyclonal antibody (15786-1-AP)

    WB analysis of human urine using 15786-1-AP

    human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using VTA1 Polyclonal antibody (15786-1-AP)

    WB analysis of rat brain using 15786-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using VTA1 Polyclonal antibody (15786-1-AP)

    IP experiment of HEK-293 using 15786-1-AP

    IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:300) with HEK-293 cells lysate 3000 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VTA1 Polyclonal antibody (15786-1-AP)

    IF Staining of HeLa using 15786-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HepG2 cells, human urine sample, K-562 cells, rat brain tissue, HSC-T6 cells, SMMC-7721 cells, mouse brain tissue, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15786-1-AP targets VTA1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VTA1 fusion protein Ag8485
Full Name Vps20-associated 1 homolog (S. cerevisiae)
Calculated Molecular Weight 307 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC006989
Gene Symbol VTA1
Gene ID (NCBI) 51534
RRIDAB_2288524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VTA1 antibody 15786-1-APDownload protocol
IF protocol for VTA1 antibody 15786-1-APDownload protocol
IP protocol for VTA1 antibody 15786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic analyses uncover a new function and mode of action for mDia2.

Authors - Tadamoto Isogai
View Article