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1-Methyladenosine Monoklonaler Antikörper

1-Methyladenosine Monoklonal Antikörper für Dot Blot, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

chemical compound

Anwendung

Dot Blot, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F2H8

Kat-Nr. : 68636-1-Ig

Synonyme

N1-methyladenosine, m1A

Filter:
  • Dot Blot experiment of RNA using 1-Methyladenosine Monoclonal antibody (68636-1-Ig)

    Dot Blot experiment of RNA using 68636-1-Ig

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose.

Geprüfte Anwendungen

Erfolgreiche Detektion in Dot BlotRNA

Empfohlene Verdünnung

AnwendungVerdünnung
DOT BLOTDOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68636-1-Ig bindet in Dot Blot, ELISA 1-Methyladenosine und zeigt Reaktivität mit chemical compound

Getestete Reaktivität chemical compound
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen PTG
Vollständiger Name 1-Methyladenosine
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf