• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

1-Methyladenosine Monoklonaler Antikörper

1-Methyladenosine Monoklonal Antikörper für Dot Blot, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

chemical compound

Anwendung

Dot Blot, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F2H8

Kat-Nr. : 68636-1-PBS

Synonyme

N1-methyladenosine, m1A

Filter:
  • Dot Blot experiment of RNA using 1-Methyladenosine Monoclonal antibody (68636-1-Ig)

    Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose. This data was developed using the same antibody clone with 68636-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68636-1-PBS bindet in Dot Blot, Indirect ELISA 1-Methyladenosine und zeigt Reaktivität mit chemical compound

Getestete Reaktivität chemical compound
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen PTG
Vollständiger Name 1-Methyladenosine
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf