14-3-3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

14-3-3 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 14503-1-AP

Synonyme

YWHAQ, 14-3-3 protein T-cell, 14-3-3 theta, 14-3-3 zeta, 14-3-3σ

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• 

  WB analysis using 14503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14503-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14503-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14503-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14503-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14503-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 14503-1-AP

  IP result of anti-14-3-3 (IP:14503-1-AP, 5ug; Detection:14503-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14503-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14-3-3 antibody (14503-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HepG2 using 14503-1-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14503-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug 14-3-3 Polyclonal antibody (14503-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Jurkat-Zellen, Maushirngewebe, Mausherzgewebe, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• RIP: See 2 publications below

Produktinformation

14503-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA 14-3-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: 14-3-3 fusion protein Ag5959
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC056867
• Gene symbol: 14-3-3 theta
• Gene ID (NCBI): 10971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publikationen

Species	Application	Title
human	WB	Neuron; Targeting 14-3-3θ-mediated TDP-43 pathology in amyotrophic lateral sclerosis and frontotemporal dementia mice; Authors - Yazi D Ke
		Int J Biol Macromol; Biofilm formation initiating rotifer-specific biopolymer and its predicted components; Authors - Zsolt Datki
human	WB	Elife; Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.; Authors - Rosalind Norkett ; KD Validated
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
mouse,human	WB,IP	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
human	WB	J Inflamm Res; PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.; Authors - Bo Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Renal Cancer cell line