14-3-3 Monoklonaler Antikörper

14-3-3 Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2E10A3

Kat-Nr. : 66061-1-Ig

Synonyme

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ

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Galerie der Validierungsdaten

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  WB analysis using 66061-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 66061-1-Ig

  IP result of anti-14-3-3(IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug.

• 

  IHC staining of human lung cancer using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat brain using 66061-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66061-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  FC experiment of HeLa using 66061-1-Ig

  1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (66061-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66061-1-Ig bindet in WB, IP, IHC, IF, FC, CoIP, ELISA 14-3-3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: 14-3-3 fusion protein Ag6055
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 31 kDa, 28 kDa
• GenBank-Zugangsnummer: BC056867
• Gene symbol: YWHAQ
• Gene ID (NCBI): 10971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publikationen

Species	Application	Title
mouse	IHC	Aging (Albany NY); In vitro and in vivo efficacy of the novel oral proteasome inhibitor NNU546 in multiple myeloma.; Authors - Hui Zhou
human	IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos
human,rat	WB,CoIP	Neurosci Res; HAP1 interacts with 14-3-3 to regulate epileptic seizure via GABAAR-mediated inhibitory synaptic transmission in pentylenetetrazole rat model.; Authors - Yuetao Wen
mouse	WB,IP	Clin Nutr; Ceramide enhanced the hepatic glucagon response through regulation of CREB activity; Authors - Jizheng Wang