14-3-3 Sigma Monoklonaler Antikörper

14-3-3 Sigma Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F10D9

Kat-Nr. : 66251-1-PBS

Synonyme

14-3-3 protein sigma, 14-3-3sigma, 3F10D9, HME1, SFN

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  WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

  Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of A-253 using 66251-1-Ig (same clone as 66251-1-PBS)

  A-253 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 Sigma antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66251-1-Ig (same clone as 66251-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66251-1-Ig (same clone as 66251-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14-3-3 Sigma antibody (66251-1-Ig, Clone: 3F10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66251-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA 14-3-3 Sigma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: 14-3-3 Sigma fusion protein Ag12644
• Vollständiger Name: stratifin
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC002995
• Gene symbol: 14-3-3 Sigma
• Gene ID (NCBI): 2810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.