14-3-3 Epsilon Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

14-3-3 Epsilon Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 11648-2-AP

Synonyme

14 3 3 protein epsilon, 14 3 3E, 14-3-3, 14-3-3 epsilon, KCIP 1, MDCR, MDS, YWHAE

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Galerie der Validierungsdaten

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  WB analysis of A375 using 11648-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11648-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 11648-2-AP

  IP Result of anti-14-3-3 epsilon (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

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  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11648-2-AP (14-3-3 epsilon antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse brain using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11648-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11648-2-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 11648-2-AP

  1X10^6 HepG2 cells were stained with 0.2ug 14-3-3 epsilon antibody (11648-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 4 publications below
• IP: See 3 publications below

Produktinformation

11648-2-AP bindet in WB, IP, IHC, IF, FC, ELISA 14-3-3 Epsilon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: 14-3-3 Epsilon fusion protein Ag2247
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Berechnetes Molekulargewicht: 255 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29-32 kDa
• GenBank-Zugangsnummer: BC000179
• Gene symbol: YWHAE
• Gene ID (NCBI): 7531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.

Publikationen

Species	Application	Title
human	WB,IF	Nat Immunol; A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.; Authors - Ying Kai Chan
human	WB	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu
human	IP	Cell Host Microbe; Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.; Authors - William Riedl
mouse	WB	EMBO J; AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.; Authors - Xin Zheng
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik
mouse	WB	Neurobiol Dis; Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models; Authors - Rodrigo Vinueza-Gavilanes

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