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ALOX15B Polyklonaler Antikörper

ALOX15B Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13073-1-PBS

Synonyme

15 lipoxygenase 2, 15-LOX-2, 15-lipoxygenase 2, 15 LOX B, 15 LOX 2

Filter:
  • Western Blot (WB) analysis of A431 cells using ALOX15B Polyclonal antibody (13073-1-AP)

    WB analysis of A431 using 13073-1-AP (same clone as 13073-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 13073-1-AP (15 Lipoxygenase 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13073-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ALOX15B Polyclonal antibody (13073-1-AP)

    WB analysis of HEK-293 using 13073-1-AP (same clone as 13073-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13073-1-AP (15 Lipoxygenase 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13073-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

13073-1-PBS bindet in WB, Indirect ELISA ALOX15B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ALOX15B fusion protein Ag3741
Vollständiger Name arachidonate 15-lipoxygenase, type B
Berechnetes Molekulargewicht 676 aa, 76 kDa
Beobachtetes Molekulargewicht 67-76 kDa
GenBank-ZugangsnummerBC035217
Gene symbol ALOX15B
Gene ID (NCBI) 247
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.