SARS-CoV-2 Envelope Protein Polyklonaler Antikörper

SARS-CoV-2 Envelope Protein Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28904-1-AP

Synonyme

COV19, COV-19, COVID19, COVID-19 E protein, E Protein, envelope protein, SARS-COV-2 E Protein, SARS-CoV-2 Envelope Protein

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  ELISA experiment of Recombinant protein using 28904-1-AP

  SARS-CoV-2 Envelope protein Antibody (28904-1-AP) tested by ELISA. SARS-CoV-2 Envelope protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Envelope protein Antibody (28904-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

28904-1-AP bindet in WB, IF, ELISA SARS-CoV-2 Envelope Protein und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Envelope Protein fusion protein Ag30690
• Vollständiger Name: COVID-19 E Protein
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: SARS-CoV-2 Envelope Protein
• Gene ID (NCBI): 43740570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Store at -20°C. Stable for one year after shipment. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Envelope protein of coronaviruses is a structural protein existing in both monomeric and homopentameric form. It is a minor component of the virus membrane though it is deemed to be important for many stages including virus infection, replication, dissemination and immune response stimulation. Sequence comparison has shown that this protein is identical to the counterparts of specific Bat and Pangolin coronavirus isolates, even though the Sars-CoV-2 sequence seems to possess specific modifications and characteristics with respect to other Sars CoVs(PMID:32596311). The SARS-CoV-2 envelope protein provide a strategy to assess cross-protection against COVID-19 (PMID: 32446902).

Publikationen

Species	Application	Title
	WB,IF	bioRxiv; Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus; Authors - Qiwang Xiang
human	IF	iScience; TLR2/NF-κB signaling in macrophage/microglia mediated COVID-pain induced by SARS-CoV-2 envelope protein; Authors - Huan Cui
human	IF	Front Cell Neurosci; The SARS-CoV-2 envelope protein disrupts barrier function in an in vitro human blood-brain barrier model; Authors - Jiahang Ju