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SARS-CoV-2 Membrane Glycoprotein Polyklonaler Antikörper

SARS-CoV-2 Membrane Glycoprotein Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28882-1-AP

Synonyme

2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein

Galerie der Validierungsdaten

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  • ELISA experiment of Recombinant protein using SARS-CoV-2 Membrane Glycoprotein Polyclonal antibo (28882-1-AP)

    ELISA experiment of Recombinant protein using 28882-1-AP

    COVID-19 M Protein Antibody (28882-1-AP) tested by ELISA. COVID-19 M Protein(101-222aa ) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of COVID-19 M Protein Antibody (28882-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

Geprüfte Anwendungen

Erfolgreiche Detektion in ELISARecombinant protein

Empfohlene Verdünnung

AnwendungVerdünnung
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

28882-1-AP bindet in , ELISA SARS-CoV-2 Membrane Glycoprotein und zeigt Reaktivität mit Virus

Getestete Reaktivität Virus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SARS-CoV-2 Membrane Glycoprotein fusion protein Ag30691
Vollständiger Name COVID-19 M Protein
GenBank-ZugangsnummerNC_045512
Gene symbol COVID-19 M Protein
Gene ID (NCBI) 43740571
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0,02% Natriumazid und 50% Glyzerin, pH 7,3 und 0,05% BSA
LagerungsbedingungenBei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).