SARS-CoV-2 Nucleocapsid Phosphoprotein Polyklonaler Antikörper

SARS-CoV-2 Nucleocapsid Phosphoprotein Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28769-1-AP

Synonyme

N protein, nucleocapsid phosphoprotein

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  WB analysis using 28769-1-AP

  SARS-CoV-2 Nucleocapsid Phosphoprotein with the domain 1-419aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28769-1-AP (2019-nCOV nucleocapsid phosphoprotein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of Recombinant protein using 28769-1-AP

  SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP) tested by ELISA.SARS-CoV-2 Nucleocapsid Phosphoprotein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

28769-1-AP bindet in WB, ELISA SARS-CoV-2 Nucleocapsid Phosphoprotein und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Vollständiger Name: COVID-19 N Protein
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules.; Authors - Yi Zheng
human	WB	Antiviral Res; RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5ʹ-untranslated region; Authors - Yongxuan Yao
human	WB	Virol J; LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19; Authors - Xinyi Kong