SARS-CoV-2 Nucleocapsid Phosphoprotein Monoklonaler Antikörper

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Virus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1B3C3

Kat-Nr. : 67666-1-Ig

Synonyme

nucleocapsid phosphoprotein, 1B3C3, sars cov 2

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  WB analysis using 67666-1-Ig

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration.

• 

  ELISA experiment of Recombinant protein using 67666-1-Ig

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

• 

  ELISA experiment of Recombinant protein using 67666-1-Ig

  Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ag30676
• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67666-1-Ig bindet in WB, ELISA SARS-CoV-2 Nucleocapsid Phosphoprotein und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Vollständiger Name: COVID-19 N Protein
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.

Publikationen

Species	Application	Title
mouse		Biosens Bioelectron; Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms.; Authors - I Novodchuk
		Viruses; Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins.; Authors - Marina Pribanić Matešić
		J Virol; Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation; Authors - Zhaohuan Wang