SARS-CoV-2 Nucleocapsid Phosphoprotein Rekombinanter Antikörper

SARS-CoV-2 Nucleocapsid Phosphoprotein Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein, Virus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

8C20

Kat-Nr. : 80027-1-RR

Synonyme

nucleocapsid phosphoprotein, sars cov 2, 8C20

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  WB analysis of Recombinant protein using 80027-1-RR

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

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  WB analysis of Recombinant protein using 80027-1-RR

  Eukaryotic expressed SARS-CoV-2 Nucleocapsid Phosphoprotein was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

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  ELISA experiment of Recombinant protein using 80027-1-RR

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 80026-1-RR and 80027-1-RR respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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  ELISA experiment of Recombinant protein using 80027-1-RR

  Sandwich ELISA was carried out by coating 80027-1-RR at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated80026-1-RR was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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  Affinity and Kinetic Characterization of 80027-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80027-1-RR against SARS-CoV-2 Nucleocapsid Phosphoprotein were performed. The affinity constant is 1.02 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Eukaryotisches Nucleocapsid-Phosphoprotein, Recombinant protein
• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

80027-1-RR bindet in WB, ELISA SARS-CoV-2 Nucleocapsid Phosphoprotein und zeigt Reaktivität mit rekombinanten Protein, Virus

• Getestete Reaktivität: rekombinanten Protein, Virus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Vollständiger Name: COVID-19 N Protein
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.

Publikationen

Species	Application	Title
human	WB	Virol Sin; The SARS-CoV-2 3CL protease inhibits pyroptosis through the cleavage of gasdermin D; Authors - Yecheng Zhang