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Phospho-4EBP1 (Thr37) Rekombinanter Antikörper

Phospho-4EBP1 (Thr37) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240535G3

Kat-Nr. : 81812-4-PBS

Synonyme

4EBP1 (Thr37), 4E-BP1 (Thr37), p-4EBP1, p-4E-BP1, p-4EBP1 (Thr37)

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-4EBP1 (Thr37) Recombinant antibody (81812-4-RR)

    WB analysis using 81812-4-RR (same clone as 81812-4-PBS)

    Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81812-4-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

81812-4-PBS bindet in WB, Indirect ELISA Phospho-4EBP1 (Thr37) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name eukaryotic translation initiation factor 4E binding protein 1
Berechnetes Molekulargewicht 118 aa, 12 kDa
Beobachtetes Molekulargewicht 15-20 kDa
GenBank-ZugangsnummerBC004459
Gene symbol EIF4EBP1
Gene ID (NCBI) 1978
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)