5 Lipoxygenase Polyklonaler Antikörper

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5 Lipoxygenase Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10021-1-PBS

Synonyme

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  WB analysis using 10021-1-Ig (same clone as 10021-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10021-1-Ig (same clone as 10021-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 10021-1-Ig (same clone as 10021-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 10021-1-Ig (same clone as 10021-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10021-1-Ig (same clone as 10021-1-PBS)

  Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10021-1-PBS bindet in WB, IF/ICC, Indirect ELISA 5 Lipoxygenase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: arachidonate 5-lipoxygenase
• Berechnetes Molekulargewicht: 674 aa, 78 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC130332
• Gene symbol: 5 Lipoxygenase
• Gene ID (NCBI): 240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

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