5 Lipoxygenase Rekombinanter Antikörper

5 Lipoxygenase Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240740A10

Rezensionen (1)

Kat-Nr. : 83794-4-RR

Synonyme

ALOX5, 5-LO, 5LO, 5-lipoxygenase, 5 LOX

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  WB analysis using 83794-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83794-4-RR (ALOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 83794-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83794-4-RR (5 Lipoxygenase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83794-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83794-4-RR against Human 5 Lipoxygenase were performed. The affinity constant is 0.392 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, humanes Plazenta-Gewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: human ovarian cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83794-4-RR bindet in WB, IHC, ELISA 5 Lipoxygenase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: 5 Lipoxygenase fusion protein Ag19503
• Vollständiger Name: arachidonate 5-lipoxygenase
• Berechnetes Molekulargewicht: 674 aa, 78 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC130332
• Gene symbol: 5 Lipoxygenase
• Gene ID (NCBI): 240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nazek (Verified Customer) (07-23-2025)

saw multiple bands

• Applications: Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SK-N-BE(2)

5 Lipoxygenase Antibody Cell culture validation (1:1000 dilution) in SK-N-BE(2) (Cat no:83794-4-RR)