CD73 Rekombinanter Antikörper

CD73 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241541D10

Kat-Nr. : 84434-3-PBS

Synonyme

5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99, Ecto-5'-nucleotidase

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  Sandwich ELISA standard curve of MP01279-4

  Sandwich ELISA standard curve of MP01279-4, Mouse CD73 Recombinant Matched Antibody Pair - PBS only. 84434-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1389. 84434-7-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP01279-4

  The mean CD73 concentration was determined to be 7.68 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  Sample test of MP01279-4

  Mouse liver and brain tissue were rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for mouse CD73 and measured 23.05 ng/mL based on a 3.3 mg/mL homogenate load, and 4.37 ng/mL based on a 2.2 mg/mL homogenate load, respectively.

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Geprüfte Anwendungen

Produktinformation

84434-3-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test CD73 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD73 fusion protein Eg1389
• Vollständiger Name: 5' nucleotidase, ecto
• Berechnetes Molekulargewicht: 64kDa
• GenBank-Zugangsnummer: NM_011851.4
• Gene symbol: Nt5e
• Gene ID (NCBI): 23959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.