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CD73 Rekombinanter Antikörper

CD73 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241541F5

Kat-Nr. : 84434-4-PBS

Synonyme

241541F5, 5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using CD73 Recombinant antibody (84434-4-RR)

    WB analysis of mouse brain using 84434-4-RR (same clone as 84434-4-PBS)

    mouse brain tissue was subjected to SDS PAGE followed by western blot with 84434-4-RR (5'-Nucleotidase/CD73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84434-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 4T1 cells using CD73 Recombinant antibody (84434-4-RR)

    IF Staining of 4T1 using 84434-4-RR (same clone as 84434-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using 5'-Nucleotidase/CD73 antibody (84434-4-RR, Clone: 241541F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84434-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84434-4-RR

    Affinity and Kinetic Characterization of 84434-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84434-4-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84434-4-PBS bindet in WB, IF/ICC, Indirect ELISA CD73 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD73 fusion protein Eg1389
Vollständiger Name 5' nucleotidase, ecto
Berechnetes Molekulargewicht64kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerNM_011851.4
Gene symbol Nt5e
Gene ID (NCBI) 23959
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).