6*His, His-Tag Rekombinanter Antikörper

6*His, His-Tag Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

FC (Intra)

Konjugation

PE Fluorescent Dye

CloneNo.

251107A1

Kat-Nr. : PE-FcA98450

Synonyme

6*His, His Tag, His Tag, 251107A1, 6*His, 6*his tag

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  FC experiment of Transfected HEK-293 using PE-FcA98450

  1x10^6 HEK-293T or transfected HEK-293T cells were intracellularly stained with 0.1 ug PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone:251107A1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

• 

  FC experiment of Transfected HEK-293 using PE-FcA98450

  1x10^6 transfected HEK-293T cells were intracellularly stained with 0.1 ug PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1) (red) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). 1x10^6 untransfected HEK-293T cells were intracellularly stained with 0.1 ug PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-FcA98450 bindet in FC (Intra) 6*His, His-Tag und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: unknow
• Vollständiger Name: 6*His, His-Tag
• Berechnetes Molekulargewicht: 1 kDa
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.