6*his,His tag Polyklonaler Antikörper
6*his,His tag Polyklonal Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
rekombinanten Protein und mehr (5)
Anwendung
WB, IP, IHC, IF, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10001-0-AP
Synonyme
"6*his,His tag Antibodies" Comparison
View side-by-side comparison of 6*his,His tag antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Recombinant protein |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 44 publications below |
| IHC | See 1 publications below |
| IF | See 7 publications below |
| IP | See 5 publications below |
| ChIP | See 1 publications below |
Produktinformation
10001-0-AP bindet in WB, IP, IHC, IF, ChIP, ELISA 6*his,His tag und zeigt Reaktivität mit rekombinanten Protein
| Getestete Reaktivität | rekombinanten Protein |
| In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Rekombinantes Protein |
| Vollständiger Name | 6*his,His tag |
| Berechnetes Molekulargewicht | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts specifically with the 6*His tag fused to recombinant protein.
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Nat Commun Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery. | ||
Nat Commun Regulation of energy homeostasis by the ubiquitin-independent REGγ proteasome. | ||
Cell Rep p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Brogan (Verified Customer) (11-09-2022) | Works very well, will buy again
|
FH Jean-Baptiste (Verified Customer) (04-21-2022) | Tested in Western blot for analysing the production in bacteria and the purification of a recombinant his-tagged protein. Worked quite well, with few unspecific bands.
 |
