6*his,His tag Rekombinanter Antikörper

6*his,His tag Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241261A7

Kat-Nr. : 84814-1-RR

Synonyme

6*His, His-Tag, 6*His, His Tag, His Tag, 6XHis, 6histag

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  WB analysis using 84814-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84814-1-RR (6*his,His tag antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 84814-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 6*his,His tag antibody (84814-1-RR, Clone: 241261A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Transfected HEK-293T using 84814-1-RR

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.25 ug 6*his,His tag Recombinant antibody (84814-1-RR, Clone:241261A7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84814-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with N-terminal 6*His Tag were performed. The affinity constant is 1.69 nM.

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  Affinity and Kinetic Characterization of 84814-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84814-1-RR against a protein with C-terminal 6*His Tag were performed. The affinity constant is 26.1 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfected HEK-293T cells
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84814-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA 6*his,His tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: 6*his,His tag
• Berechnetes Molekulargewicht: 1 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.