A2BP1 Polyklonaler Antikörper

A2BP1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22647-1-AP

Synonyme

A2BP, A2BP1, ataxin 2 binding protein 1, Fox 1 homolog A, FOX1, HRNBP1, RBFOX1

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Galerie der Validierungsdaten

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  WB analysis using 22647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22647-1-AP (A2BP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22647-1-AP

  IP Result of anti-A2BP1 (IP:22647-1-AP, 4ug; Detection:22647-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 22647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 22647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 22647-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 22647-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 22647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22647-1-AP (A2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 22647-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22647-1-AP (A2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, mouse liver, mouse brain
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• RIP: See 1 publications below

Produktinformation

22647-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA A2BP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: A2BP1 fusion protein Ag18527
• Vollständiger Name: ataxin 2-binding protein 1
• Berechnetes Molekulargewicht: 395 aa, 42 kDa
• Beobachtetes Molekulargewicht: 45-70 kDa
• GenBank-Zugangsnummer: BC113691
• Gene symbol: A2BP1
• Gene ID (NCBI): 54715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.A2BP1 has two mouse isoforms (A2BP1-A016 and -A030) exogenously expressed in COS7 cells showed a molecular mass of 65 kDa, although the calculated molecular masses of A016 and A030 are 40 and 42 kDa, respectively in the course of cell biological study.

Publikationen

Species	Application	Title
human	WB,RIP	Mol Ther Oncolytics; RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.; Authors - Shuyuan Shen ; KD Validated