A4GALT Polyklonaler Antikörper

A4GALT Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12392-1-AP

Synonyme

A4GALT, A4GALT1, CD77 synthase, Gb3 synthase

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 12392-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12392-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12392-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12392-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12392-1-AP bindet in WB, IHC, IF, ELISA A4GALT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: A4GALT fusion protein Ag3069
• Vollständiger Name: alpha 1,4-galactosyltransferase
• Berechnetes Molekulargewicht: 353 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50-52 kDa
• GenBank-Zugangsnummer: BC017068
• Gene symbol: A4GALT
• Gene ID (NCBI): 53947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.