A4GALT Polyklonaler Antikörper

A4GALT Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12392-1-PBS

Synonyme

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  WB analysis of RAW 264.7 using 12392-1-AP (same clone as 12392-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12392-1-AP (same clone as 12392-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 12392-1-AP (same clone as 12392-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12392-1-AP (same clone as 12392-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12392-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA A4GALT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: A4GALT fusion protein Ag3069
• Vollständiger Name: alpha 1,4-galactosyltransferase
• Berechnetes Molekulargewicht: 353 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50-52 kDa
• GenBank-Zugangsnummer: BC017068
• Gene symbol: A4GALT
• Gene ID (NCBI): 53947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.