A4GNT Polyklonaler Antikörper

A4GNT Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22670-1-PBS

Synonyme

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

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  WB analysis using 22670-1-AP (same clone as 22670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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  WB analysis of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 22670-1-AP (same clone as 22670-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 22670-1-AP (same clone as 22670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22670-1-PBS bindet in WB, IHC, Indirect ELISA A4GNT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: A4GNT fusion protein Ag18432
• Vollständiger Name: alpha-1,4-N-acetylglucosaminyltransferase
• Berechnetes Molekulargewicht: 340 aa, 39 kDa
• Beobachtetes Molekulargewicht: 34-37 kDa
• GenBank-Zugangsnummer: BC119639
• Gene symbol: A4GNT
• Gene ID (NCBI): 51146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.