AACS Polyklonaler Antikörper

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AACS Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13815-1-PBS

Synonyme

Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1

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  WB analysis of mouse heart using 13815-1-AP (same clone as 13815-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13815-1-AP (same clone as 13815-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13815-1-AP (same clone as 13815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13815-1-AP (AACS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13815-1-AP (same clone as 13815-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13815-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13815-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AACS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AACS fusion protein Ag4899
• Vollständiger Name: acetoacetyl-CoA synthetase
• Berechnetes Molekulargewicht: 672 aa, 75 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 30 kDa
• GenBank-Zugangsnummer: BC040490
• Gene symbol: AACS
• Gene ID (NCBI): 65985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.