AADAT Polyklonaler Antikörper

AADAT Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 13031-1-AP

Synonyme

2 aminoadipate transaminase, 2-aminoadipate aminotransferase, 2-aminoadipate transaminase, Alpha-aminoadipate aminotransferase, aminoadipate aminotransferase

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  IHC staining of mouse cerebellum using 13031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13031-1-AP (AADAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13031-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13031-1-AP (AADAT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13031-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13031-1-AP (AADAT antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

13031-1-AP bindet in IHC, IF, ELISA AADAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AADAT fusion protein Ag3676
• Vollständiger Name: aminoadipate aminotransferase
• Berechnetes Molekulargewicht: 429 aa, 48 kDa
• GenBank-Zugangsnummer: BC031068
• Gene symbol: AADAT
• Gene ID (NCBI): 51166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AADAT (alpha-aminoadipate aminotransferase), also known as KAT (kynurenine aminotransferase) II, is a primary enzyme in the brain for catalysing the transamination of kynurenine to KYNA (kynurenic acid). KYNA is the only known endogenous antagonist of the N-methyl-D-aspartate receptor. AADAT is also involved in lysine metabolism by catalyzing the transamination of aminoadipate to a-ketoadipate (PMID: 17024659, 18056995). The human AADAT enzyme is also predicted to contain a mitochondrial cleavage signal, suggesting that AADAT is a mitochondrial protein (PMID: 19826765).

Publikationen

Species	Application	Title
mouse	IF	Brain Struct Funct; Astrocytic and neuronal localization of kynurenine aminotransferase-2 in the adult mouse brain.; Authors - Judit Herédi
mouse	IHC	Neurochem Int; Species-specific neuronal localization of kynurenine aminotransferase-2 in the mouse cerebellum.; Authors - Emma Balog
		J Clin Med; Impaired Skeletal Muscle Kynurenine Metabolism in Patients with Chronic Obstructive Pulmonary Disease.; Authors - Harry R Gosker
monkey	IF	bioRxiv; Kynurenic acid inflammatory signaling expands in primates and impairs prefrontal cortical cognition; Authors - Shengtao Yang
mouse	IHC,IF	Cell Commun Signal; Helicobacter pylori promotes gastric intestinal metaplasia through activation of IRF3-mediated kynurenine pathway; Authors - Xinhua Liang