AAMP Polyklonaler Antikörper

AAMP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 21220-1-AP

Synonyme

Angio-associated migratory cell protein

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  WB analysis using 21220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21220-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21220-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21220-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21220-1-AP (AAMP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 21220-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21220-1-AP (AAMP antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 21220-1-AP

  Immunofluorescent analysis of MCF-7 cells using 21220-1-AP (AAMP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Herzgewebe, MCF-7-Zellen, Mausnierengewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

21220-1-AP bindet in WB, IHC, IF/ICC, ELISA AAMP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AAMP fusion protein Ag15655
• Vollständiger Name: angio-associated, migratory cell protein
• Berechnetes Molekulargewicht: 434 aa, 47 kDa
• Beobachtetes Molekulargewicht: 52-58 kDa
• GenBank-Zugangsnummer: BC039866
• Gene symbol: AAMP
• Gene ID (NCBI): 14
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IF	Exp Ther Med; MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BB.; Authors - Xiaogao Wang