AlaRS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AlaRS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17394-1-AP

Synonyme

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 17394-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17394-1-AP

  WB result of AlaRS antibody (17394-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HepG2 cells.

• 

  WB analysis of Jurkat using 17394-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17394-1-AP

  IP Result of anti-AlaRS (IP:17394-1-AP, 4ug; Detection:17394-1-AP 1:500) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human liver using 17394-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17394-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 17394-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 17394-1-AP (AlaRS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17394-1-AP bindet in WB, IP, IHC, IF, ELISA AlaRS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AlaRS fusion protein Ag11294
• Vollständiger Name: alanyl-tRNA synthetase
• Berechnetes Molekulargewicht: 968 aa, 107 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC011451
• Gene symbol: AARS
• Gene ID (NCBI): 16
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Publikationen

Species	Application	Title
human	WB,IHC	Nat Commun; Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes; Authors - Lingling Li