AlaRS Polyklonaler Antikörper

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• KD/KO Validated

AlaRS Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 17394-1-AP

Synonyme

AARS, AARS1, Alanine--tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase, EC:6.1.1.7

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  WB analysis using 17394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17394-1-AP

  WB result of AlaRS antibody (17394-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HepG2 cells.

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  WB analysis of Jurkat using 17394-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17394-1-AP (AlaRS antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 17394-1-AP

  IP result of anti-AlaRS (IP:17394-1-AP, 4ug; Detection:17394-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of mouse liver using 17394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17394-1-AP (AlaRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17394-1-AP (AlaRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17394-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17394-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17394-1-AP (AlaRS antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 17394-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (17394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, L02-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maushirngewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

17394-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA AlaRS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AlaRS fusion protein Ag11294
• Vollständiger Name: alanyl-tRNA synthetase
• Berechnetes Molekulargewicht: 968 aa, 107 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC011451
• Gene symbol: AlaRS
• Gene ID (NCBI): 16
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Publikationen

Species	Application	Title
human	WB,IHC	Nat Commun; Comprehensive proteogenomic characterization of early duodenal cancer reveals the carcinogenesis tracks of different subtypes; Authors - Lingling Li
human	CoIP	Cancer Lett; K90 lactylation orchestrates YAP nuclear sequestration by impairing binding with exportin CRM1 and enhances HCC malignancy; Authors - Li Wang
human	WB	Sci Adv; Infection-induced lysine lactylation enables herpesvirus immune evasion; Authors - Matthew D Tyl ; KD Validated
human	WB	Cell Discov; NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance; Authors - Jinlong Lin
human	WB,CoIP	Cell Rep; High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy; Authors - Zhuo Xing ; KD Validated
mouse	WB	Acta Histochem; AARS1-mediated Osterix lactylation promotes its transcriptional activity during osteoblast differentiation; Authors - Feige Nian ; KD Validated