AARS2 Polyklonaler Antikörper

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• KD/KO Validated

AARS2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 22696-1-AP

Synonyme

AARSL, Alanine tRNA ligase, Alanine--tRNA ligase, mitochondrial, Alanyl tRNA synthetase like, Alanyl-tRNA synthetase

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  WB analysis of K-562 using 22696-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22696-1-AP (AARS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 22696-1-AP

  IP result of anti-AARS2 (IP:22696-1-AP, 4ug; Detection:22696-1-AP 1:10000) with K-562 cells lysate 1600 ug.

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  IHC staining of human lung cancer using 22696-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 22696-1-AP (AARS2 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 22696-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 22696-1-AP (AARS2 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

22696-1-AP bindet in WB, IHC, IP, ELISA AARS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AARS2 fusion protein Ag18539
• Vollständiger Name: alanyl-tRNA synthetase 2, mitochondrial (putative)
• Berechnetes Molekulargewicht: 985 aa, 107 kDa
• Beobachtetes Molekulargewicht: 105-115 kDa
• GenBank-Zugangsnummer: BC131728
• Gene symbol: AARS2
• Gene ID (NCBI): 57505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao ; KO Validated
rat	WB	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
human	WB	Cancer Med; AARS2 as a novel biomarker for prognosis and its molecular characterization in pan-cancer; Authors - Long Liu