AATF Polyklonaler Antikörper

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AATF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10282-1-PBS

Synonyme

Apoptosis-antagonizing transcription factor, CHE 1, CHE1, DED, HSPC277

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  WB analysis using 10282-1-AP (same clone as 10282-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10282-1-AP (same clone as 10282-1-PBS)

  WB result of AATF antibody (10282-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10282-1-AP (same clone as 10282-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 10282-1-AP (same clone as 10282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10282-1-AP (AATF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10282-1-AP (same clone as 10282-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using AATF antibody (10282-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10282-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10282-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AATF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AATF fusion protein Ag0195
• Vollständiger Name: apoptosis antagonizing transcription factor
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 80-95 kDa
• GenBank-Zugangsnummer: BC000591
• Gene symbol: AATF
• Gene ID (NCBI): 26574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

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