ABCA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ABCA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 26564-1-AP

Synonyme

ABC1, ABC-1, ATP-binding cassette 1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1

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  WB analysis using 26564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26564-1-AP (ABCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 26564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26564-1-AP (ABCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 26564-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26564-1-AP (ABCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

26564-1-AP bindet in WB, IHC, IF, ELISA ABCA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCA1 fusion protein Ag24118
• Vollständiger Name: ATP-binding cassette, sub-family A (ABC1), member 1
• Berechnetes Molekulargewicht: 2261 aa, 254 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC141816
• Gene symbol: ABCA1
• Gene ID (NCBI): 19
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABCA, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway.

Publikationen

Species	Application	Title
mouse	WB	Clin Transl Med; Tumour-associated macrophage-derived DOCK7-enriched extracellular vesicles drive tumour metastasis in colorectal cancer via the RAC1/ABCA1 axis; Authors - Weiwei Chen ; KD Validated
mouse,human	WB,IHC	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu
mouse	WB,IHC	Atherosclerosis; Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B; Authors - Yuanyuan Li
human	IF	Front Cardiovasc Med; miR-3529-3p/ABCA1 axis regulates smooth muscle cell homeostasis by enhancing inflammation via JAK2/STAT3 pathway; Authors - Tingyu Wang
human	IHC	Biofactors; Investigating the dysregulation of genes associated with glucose and lipid metabolism in gastric cancer and their influence on immunity and prognosis; Authors - Yan Li
mouse	WB	Drug Des Devel Ther; Baolier Capsule's Secret Weapon: Piperine Boosts Cholesterol Excretion to Combat Atherosclerosis; Authors - Mengqiu Wei