ABCB7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ABCB7 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11158-1-AP

Synonyme

ABC transporter 7 protein, ABC7, ABCB7, ASAT, Atm1p, EST140535

---

• 

  WB analysis of mouse liver using 11158-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11158-1-AP (ABCB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11158-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11158-1-AP (ABCB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11158-1-AP

  IP result of anti-ABCB7 (IP:11158-1-AP, 4ug; Detection:11158-1-AP 1:500) with mouse liver tissue lysate 4000ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

11158-1-AP bindet in WB, IP, ELISA ABCB7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCB7 fusion protein Ag1544
• Vollständiger Name: ATP-binding cassette, sub-family B (MDR/TAP), member 7
• Berechnetes Molekulargewicht: 753 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC006323
• Gene symbol: ABCB7
• Gene ID (NCBI): 22
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCB7 belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCB7 exports glutathione-coordinated iron-sulfur clusters from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur-containing proteins and participates in iron homeostasis(PMID: 33157103, 17192393). ABCB7 structure and mutation causing X- linked sideroblastic anemia and ataxia (XLSA/A) with disruption of cytosolic iron-sulfur protein maturation (PMID: 10196363,11050011).

Publikationen

Species	Application	Title
human	WB	Elife; Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8.; Authors - Hsiang-Chun Chang
human	WB	Arch Biochem Biophys; Knockdown of ABCB7 inhibits esophageal cancer progression by inhibiting the TGF-β/Smad signaling; Authors - Shiyun Feng ; KD Validated
human	WB	Mol Cell; Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis; Authors - Xiaojian Shi