MRP1 Monoklonaler Antikörper

MRP1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

CloneNo.

1G4A2

Kat-Nr. : 67228-1-Ig

Synonyme

ABCC1, 1G4A2, MRP1/ABCC1

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  WB analysis using 67228-1-Ig

  Non-boil and boiled HepG2 and A549 cell lysates were subjected to SDS PAGE followed by western blot with 67228-1-Ig (ABCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Left panel was exposed for 20 sec. and right panel was exposed for 1 min.

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  IF Staining of A549 using 67228-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MRP1 antibody (67228-1-Ig, Clone: 1G4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochte HepG2-Zellen, unboiled A549 cells
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

67228-1-Ig bindet in WB, IHC, IF/ICC, ELISA MRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MRP1 fusion protein Ag27048
• Vollständiger Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 1
• Berechnetes Molekulargewicht: 172 kDa
• Beobachtetes Molekulargewicht: 180-190 kDa
• GenBank-Zugangsnummer: NM_004996
• Gene symbol: MRP1
• Gene ID (NCBI): 4363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Multidrug resistant-associated protein 1 (MRP1; ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.

Publikationen

Species	Application	Title
human	WB	Environ Pollut; Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.; Authors - Jianqiang Zhu
human	WB	Cancers (Basel); NCAPG2 Maintains Cancer Stemness and Promotes Erlotinib Resistance in Lung Adenocarcinoma; Authors - Shiyao Jiang
human	WB	Commun Chem; Exploring bat-inspired cyclic tryptophan diketopiperazines as ABCB1 Inhibitors; Authors - Javier Yu Peng Koh ; KD Validated
human	WB	Front Cell Dev Biol; Apatinib Suppresses Gastric Cancer Stem Cells Properties by Inhibiting the Sonic Hedgehog Pathway.; Authors - Wanshuang Cao
human	WB	Transl Oncol; Pan-cancer analysis of ABCC1 as a potential prognostic and immunological biomarker; Authors - Tiantian Wang
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng