ABCC5 Polyklonaler Antikörper

ABCC5 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 19503-1-AP

Synonyme

ABC33, ABCC5, MOAT C, MOATC, MRP5, pABC11, SMRP

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Galerie der Validierungsdaten

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  IHC staining of human ovary tumor using 19503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19503-1-AP (ABCC5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 19503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19503-1-AP (ABCC5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 19503-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19503-1-AP (MRP5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 19503-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19503-1-AP (MRP5 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

19503-1-AP bindet in WB, IHC, ELISA ABCC5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• Berechnetes Molekulargewicht: 161 kDa
• GenBank-Zugangsnummer: NM_005688
• Gene symbol: ABCC5
• Gene ID (NCBI): 10057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCC5, also named as MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).

Publikationen

Species	Application	Title
human	IHC	Front Oncol; Screening of co-pathogenic genes of non-alcoholic fatty liver disease and hepatocellular carcinoma; Authors - Ting Chen
human	IHC	Front Genet; Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma.; Authors - Xin Zhou
human	WB	Oncol Lett; Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells.; Authors - Hao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yu Siong (Verified Customer) (03-01-2019)

Multiple bands appeared, non-specific binding, bands did not appear at exact molecular size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Porcine brain endothelial cells

ABCC5 Antibody Western Blot, validation (1:200 dilution) in Porcine brain endothelial cells (Cat no:19503-1-AP)