ABCC5 Rekombinanter Antikörper

ABCC5 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241380G1

Kat-Nr. : 84204-5-PBS

Synonyme

MRP5, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5, ABCC 5

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  WB analysis using 84204-5-RR (same clone as 84204-5-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84204-5-RR (ABCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 84204-5-RR (same clone as 84204-5-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 84204-5-RR (ABCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84204-5-RR (same clone as 84204-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84204-5-RR (ABCC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84204-5-RR (same clone as 84204-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84204-5-RR (ABCC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 84204-5-RR (same clone as 84204-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABCC5 antibody (84204-5-RR, Clone: 241380G1 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84204-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84204-5-RR against Human ABCC5 were performed. The affinity constant is 2.18 nM.

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Geprüfte Anwendungen

Produktinformation

84204-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ABCC5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ABCC5 fusion protein Ag31680
• Vollständiger Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• Berechnetes Molekulargewicht: 161 kDa
• Beobachtetes Molekulargewicht: 161-200 kDa
• GenBank-Zugangsnummer: NM_005688
• Gene symbol: ABCC5
• Gene ID (NCBI): 10057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCC5, also named MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercaptopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site are unclear. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).