ABCD1 Rekombinanter Antikörper

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• KD/KO Validated

ABCD1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230226E12

Kat-Nr. : 83038-4-PBS

Synonyme

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

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  WB analysis of HeLa using 83038-4-RR (same clone as 83038-4-PBS)

  WB result of ABCD1 antibody (83038-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCD1 transfected HeLa cells. This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  WB analysis using 83038-4-RR (same clone as 83038-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83038-4-RR (ABCD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83038-4-RR (same clone as 83038-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABCD1 antibody (83038-4-RR, Clone: 230226E12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83038-4-RR (same clone as 83038-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ABCD1 Recombinant antibody (83038-4-RR, Clone:230226E12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83038-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83038-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83038-4-RR against Human ABCD1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83038-4-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA ABCD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ABCD1 fusion protein Ag0453
• Vollständiger Name: ATP-binding cassette, sub-family D (ALD), member 1
• Berechnetes Molekulargewicht: 745 aa, 83 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC025358
• Gene symbol: ABCD1
• Gene ID (NCBI): 215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.