ABCD3 Monoklonaler Antikörper

ABCD3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1G7G5

Rezensionen (1)

Kat-Nr. : 66697-1-Ig

Synonyme

ABC43, ABCD3, PMP70, PXMP1

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Galerie der Validierungsdaten

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  WB analysis using 66697-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66697-1-Ig (ABCD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Caco-2-Zellen, HepG2-Zellen, HT-29-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66697-1-Ig bindet in WB, ELISA ABCD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG3
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ABCD3 fusion protein Ag24760
• Vollständiger Name: ATP-binding cassette, sub-family D (ALD), member 3
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC068509
• Gene symbol: ABCD3
• Gene ID (NCBI): 5825
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Life Sci Alliance; Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors; Authors - Nnejiuwa U Ibe
human	WB	Haematologica; Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.; Authors - Nunziata Maio

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PAULA (Verified Customer) (07-12-2023)

I tested this ABCD3 antibody at two dilutions on THP1 cells (20ug cell lysate) and I saw a nice band around 70 kDa, although some non-specific bands were also detected. Dilution 1:1000 showed similar results to 1:500 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000, 1:500
• Cell Tissue Type: THP1 cell line

ABCD3 Antibody Western Blot validation (1:1000, 1:500 dilution) in THP1 cell line (Cat no:66697-1-Ig)