ABCF2 Polyklonaler Antikörper

ABCF2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 10226-1-AP

Synonyme

ABC28, ATP-binding cassette sub-family F member 2, HUSSY18, HUSSY-18, Iron-inhibited ABC transporter 2

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  WB analysis of NIH/3T3 using 10226-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10226-1-AP (ABCF2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10226-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10226-1-AP (ABCF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10226-1-AP

  IP result of anti-ABCF2 (IP:10226-1-AP, 4ug; Detection:10226-1-AP 1:300) with HeLa cells lysate 3000 ug.

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  IHC staining of human colon cancer using 10226-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10226-1-AP (ABCF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ABCF2 antibody (10226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

10226-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ABCF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCF2 fusion protein Ag0310
• Vollständiger Name: ATP-binding cassette, sub-family F (GCN20), member 2
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC001661
• Gene symbol: ABCF2
• Gene ID (NCBI): 10061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCF2 (ABC28, M-ABC1, HUSSY-18, EST133090, DKFZp586K1823) is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins are composed of transmembrane domains (TMDs), and nucleotide binding domains (NBDs, or ATP-binding cassettes, ABSs). Based on their sequence similarity scores, the members of the human ABC protein family can be grouped into eight subfamilies, including the MDR/TAP, the ALD, the MRP/CFTR, the ABC1, the White, the RNAseL inhibitor, the ANSA, and the GCN20 subfamilies. ABCF2 is a member of the GCN20 subfamily. ABC proteins transport various molecules across extra- and intracellular membranes.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.; Authors - Erika C Urdaneta
	IHC	Elife; DMRT1 is a testis-determining gene in rabbits and is also essential for female fertility; Authors - Emilie Dujardin

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