ABHD5 Monoklonaler Antikörper

ABHD5 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B12C2

Kat-Nr. : 67779-1-PBS

Synonyme

ABHD5, CDS, CGI58, IECN2, NCIE2

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  WB analysis using 67779-1-Ig (same clone as 67779-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67779-1-Ig (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67779-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67779-1-Ig (same clone as 67779-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ABHD5 antibody (67779-1-Ig, Clone: 2B12C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67779-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67779-1-PBS bindet in WB, IF/ICC, Indirect ELISA ABHD5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ABHD5 fusion protein Ag30256
• Vollständiger Name: abhydrolase domain containing 5
• Berechnetes Molekulargewicht: 349 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC021958
• Gene symbol: ABHD5
• Gene ID (NCBI): 51099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).