ABI1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ABI1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 27387-1-AP

Synonyme

Abelson interactor 1, ABI 1, ABI1, Abl binding protein 4, abl interactor 1, AblBP4, E3B1, Eps8 binding protein, Nap1 binding protein, NAP1BP, SSH3BP, SSH3BP1

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  WB analysis using 27387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27387-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 27387-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27387-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27387-1-AP (ABI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skin using 27387-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 27387-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27387-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27387-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27387-1-AP (ABI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, C2C12-Zellen, HEK 293-Zellen, HEK-293T-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maushautgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

27387-1-AP bindet in WB, IHC, IF, CoIP, ELISA ABI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABI1 fusion protein Ag26537
• Vollständiger Name: abl-interactor 1
• Berechnetes Molekulargewicht: 507 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC024254
• Gene symbol: ABI1
• Gene ID (NCBI): 10006
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Ovarian Res; Modified screening of MYC promotor region elements using the CRISPR library in ovarian cancer; Authors - Akiko Yamamoto
human	WB	J Psychiatr Res; Circulating miR-30e-3p induces disruption of neurite development in SH-SY5Y cells by targeting ABI1, a novel biomarker for schizophrenia; Authors - Mengdi Jin ; KD Validated
human	WB,IF,CoIP	Transl Oncol; CBLC promotes the development of colorectal cancer by promoting ABI1 degradation to activate the ERK signaling pathway; Authors - Zhan Li