ABI3 Polyklonaler Antikörper

ABI3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16227-1-AP

Synonyme

SSH3BP3, New molecule including SH3, Nesh, ABI gene family member 3, ABI family, member 3

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  WB analysis using 16227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16227-1-AP (ABI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 16227-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16227-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 16227-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ABI3 antibody (16227-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, SK-N-SH cells
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16227-1-AP bindet in WB, IHC, ELISA ABI3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABI3 fusion protein Ag8164
• Vollständiger Name: ABI family, member 3
• Berechnetes Molekulargewicht: 366 aa, 39 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC007780
• Gene symbol: ABI3
• Gene ID (NCBI): 51225
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABI family member 3 belongs to the ABL Interactors (ABI) family of proteins, which also includes ABI1 and ABI2. ABI proteins are a class of cytoplasmic molecular junction proteins originally identified as binding chaperones for Abl kinase. The ABI3 gene is thought to be an important regulator of microglia during Alzheimer's disease (AD), and its S209F variant increases the risk of developing AD. Moreover, ABI3 plays an important role in suppressing tumor growth and progression.